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Registros recuperados: 65
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Temperature and Food Influence Shell Growth and Mantle Gene Expression of Shell Matrix Proteins in the Pearl Oyster Pinctada margaritifera ArchiMer
Joubert, Caroline; Linard, Clementine; Le Moullac, Gilles; Soyez, Claude; Saulnier, Denis; Teaniniuraitemoana, Vaihiti; Ky, Chin-long; Gueguen, Yannick.
In this study, we analyzed the combined effect of microalgal concentration and temperature on the shell growth of the bivalve Pinctada margaritifera and the molecular mechanisms underlying this biomineralization process. Shell growth was measured after two months of rearing in experimental conditions, using calcein staining of the calcified structures. Molecular mechanisms were studied though the expression of 11 genes encoding proteins implicated in the biomineralization process, which was assessed in the mantle. We showed that shell growth is influenced by both microalgal concentration and temperature, and that these environmental factors also regulate the expression of most of the genes studied. Gene expression measurement of shell matrix protein...
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Ano: 2014 URL: http://archimer.ifremer.fr/doc/00210/32095/30535.pdf
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Toxic effects of leachates from plastic pearl-farming gear on embryo-larval development in the pearl oyster Pinctada margaritifera ArchiMer
Gardon, Tony; Huvet, Arnaud; Paul-pont, Ika; Cassone, Anne-laure; Koua, Manaarii Sham; Soyez, Claude; Jezequel, Ronan; Receveur, Justine; Le Moullac, Gilles.
Pearl-farming leads to significant plastic pollution in French Polynesia (FP) as the end of life of most farming gear is currently poorly managed. Plastic debris released into the aquatic environment accumulates, with potentially detrimental effects on the lagoon ecosystem and pearl oyster Pinctada margaritifera, a species of ecological, commercial and social value. Here, we tested the effects of leachates from new (N) and aged (A) plastic pearl-farming gear (spat collector and synthetic rope) obtained after 24 h and 120 h incubation, on the embryo-larval development of the pearl oyster using an in-vitro assay. Embryos were exposed for 24 h and 48 h to a negative control (0) and the leachate from 0.1, 1, 10 and 100 g of plastic. L−1. After 24 h exposure to...
Tipo: Text Palavras-chave: Aquaculture; Synthetic materials; Hazardous chemicals; Toxicity; Marine bivalve; Early development.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00627/73917/73257.pdf
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Transcriptional regulation of pyruvate kinase and phosphoenolpyruvate carboxykinase in the adductor muscle of the oyster Crassostrea gigas during prolonged hypoxia ArchiMer
Le Moullac, Gilles; Bacca, Helene; Huvet, Arnaud; Moal, Jeanne; Pouvreau, Stephane; Van Wormhoudt, A.
The response of Crassostrea gigas to prolonged hypoxia was investigated for the first time by analyzing the metabolic branch point formed by pyruvate kinase (PK) and phosphoenolpyruvate carboxykinase (PEPCK). PK and PEPCK cDNAs were cloned and sequenced. The main functional domains of the PK sequence, such as the binding sites for ADP/ATP and phosphoenolpyruvate (PEP), were identified whereas the PEPCK sequence showed the specific domain to bind PEP in addition to the kinase-1 and kinase-2 motifs to bind guanosine triphosphate (GTP) and Mg2+, specific for all PEPCKs. A C-terminal extension was detected for the first time in eukaryota PK. Separation of mitochondrial and cytosolic fraction showed that more than 92% of the PEPCK enzyme activity was cytosolic...
Tipo: Text Palavras-chave: Succinate alanine carboxykinase phosphoenolpyruvate pyruvate kinase hypoxia Oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2683.pdf
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Transcriptomic response of the Pacific oyster Crassostrea gigas to hypoxia ArchiMer
Sussarellu, Rossana; Fabioux, Caroline; Le Moullac, Gilles; Fleury, Elodie; Moraga, Dario.
Marine intertidal organisms commonly face hypoxic stress during low tide emersion; moreover, eutrophic conditions and sediment nearness could lead to hypoxic phenomena; it is indeed important to understand the molecular processes involved in the response to hypoxia. In this study the molecular response of the Pacific oyster Crassostrea gigas to prolonged hypoxia (2 mg O-2 L-1 for 20 d) was investigated under experimental conditions. A transcriptomic approach was employed using a cDNA microarray of 9058 C. gigas clones to highlight the genetic expression patterns of the Pacific oyster under hypoxic conditions. Lines of oysters resistant (R) and susceptible (S) to summer mortality were used in this study. ANOVA analysis was used to identify the genes...
Tipo: Text Palavras-chave: Mollusca; Hypoxia; CDNA microarray; Gene expression; Oxidative stress; Respiratory chain.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00027/13799/11053.pdf
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Yes, it turns: experimental evidence of pearl rotation during its formation ArchiMer
Gueguen, Yannick; Czorlich, Yann; Mastail, Max; Le Tohic, Bruno; Defay, Didier; Lyonnard, Pierre; Marigliano, Damien; Gauthier, Jean-pierre; Bari, Hubert; Lo, Cedrik; Chabrier, Sebastien; Le Moullac, Gilles.
Cultured pearls are human creations formed by inserting a nucleus and a small piece of mantle tissue into a living shelled mollusc, usually a pearl oyster. Although many pearl observations intuitively suggest a possible rotation of the nucleated pearl inside the oyster, no experimental demonstration of such a movement has ever been done. This can be explained by the difficulty of observation of such a phenomenon in the tissues of a living animal. To investigate this question of pearl rotation, a magnetometer system was specifically engineered to register magnetic field variations with magnetic sensors from movements of a magnetic nucleus inserted in the pearl oyster. We demonstrated that a continuous movement of the nucleus inside the oyster starts after a...
Tipo: Text Palavras-chave: Pearl oyster; Pearl rotation; Biomineralization.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00275/38598/37127.pdf
Registros recuperados: 65
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